Born on 21st March,1978, Rani Mukerji comes from a film-oriented family of Bengali origin. Her father Ram Mukerji is a retired director and one of the founders of 'Filmalaya Studios'. Her mother Krishna was a playback singer. Her brother Raja Mukerji is a film producer, now turned director. Her maternal aunt, Debashree Roy, was a Bengali-film actress and her cousin Kajol is a popular Bollywood actress. Mukerji is a trained Odissi dancer, and began learning the dance in her tenth grade. She studied at Maneckjee Cooper High School in Juhu, and later enrolled at Mithibai College in Mumbai.
While in school at the age of 14, Mukerji did a cameo role in her father's Bengali film Biyar Phool (1992). In 1994, Salim Akhtar, a family friend, offered her a film called Aa Gale Lag Jaa (1994) but her father was against it as she was too young. Mukerji turned down the offer and Urmila Matondkar was cast instead.
Two years later, Salim Akhtar came up with another offer and insisted on her playing the protagonist of Raja Ki Ayegi Baraat (1997). After a long discussion with her father, she agreed to do the film. When the film was unsuccessful at the box office, Mukerji returned to college to complete her education. However she returned to films with Ghulam opposite Aamir Khan and Karan Johar's romantic film Kuch Kuch Hota Hai which is her biggest commercial success so far, and won a Filmfare Best Supporting Actress Award for her performance in the film. She later starred in several films, most of which fared below expectations, but in 2002 she re-invented her image with the critically acclaimed drama Saathiya opposite then new comer Vivek Oberoi, for which she received numerous awards and nominations. Mukerji's first 2003 release was Aziz Mirza's drama Chalte Chalte opposite Shahrukh Khan. The film saw her play a similar role to that of Saathiya, and she received her second nomination for Filmfare Best Actress Award. In 2004, her performances in the hit Hum Tum and the critically acclaimed Yuva earned her the Best Actress and the Best Supporting Actress awards at the Filmfare ceremony, making her the first actress to win two major awards in the same year. She later received unanimous praise for her portrayal of a blind, deaf and mute woman in Black (2005), for which she garnered numerous major awards. Mukerji has since established herself as a leading actress of Hindi cinema. Rani went on to do films like Karan Johar's Kabhi Alvida Na Kehna and B.R. Chopra's Baabul in 2006.
In 2007, Rani was seen in the films Ta Ra Rum Pum, Pradeep Sarkar's drama Laaga Chunari Mein Daag and Sanjay Leela Bhansali's Saawariya which were critical and commercial failures of her career. Mukerji's role in Kunal Kohli's Thoda Pyaar Thoda Magic, received positive reviews from critics; however, the film failed to do well at the box office. Her most recent release, Dil Bole Hadippa was screened at the Toronto International Film Festival, under the Yash Raj Films banner and performed averagely at the box office. Rani was seen in a bold and beautiful character Meera in Rajkumar Gupta's movie No One Killed Jessica. The film is believed to be based on the controversial Jessica Lal murder case.
In 2012, she was seen in the film 'Aaiyya' opposite a South actor and in 'Talaash' opposite Aamir Khan.